Course

Methods in Biotechnology (BIO520)

Practical course in PCR, plasmid isolation, restrictions enzymes, transformation, production of recombinant protein, cell and tissue culture.


Dette er emnebeskrivelsen for studieåret 2019-2020. Merk at det kan komme endringer.

See course description and exam/assesment information for this semester (2024-2025)

Semesters

Fakta

Emnekode

BIO520

Vekting (stp)

10

Semester undervisningsstart

Autumn

Undervisningsspråk

English

Antall semestre

1

Vurderingssemester

Autumn

Timeplan

Vis timeplan

Content

Practical course in protein purification, PCR, plasmid isolation, restrictions enzymes, transformation, cell and tissue culture.

Learning outcome

The student will acquire basic experience on PCR, transformation of bacteria, isolation of plasmids, production of recombinant protein, purification and detection of the protein product by immunological methods. The student will know how to work under axenic conditions, and grow seedlings in vitro. Furthermore, the student will know how to extract and measure pigments. The student will know basics in signal transduction (kinases, phosphatases). The student will have training in writing a scientific report.

Forkunnskapskrav

HSE-course for master students (TN501)

Anbefalte forkunnskaper

biochemistry and cell biology

Exam

Form of assessment Weight Duration Marks Aid Exam system Withdrawal deadline Exam date
Written exam 1/1 4 Hours Letter grades None permitted Inspera assessment — —


Vilkår for å gå opp til eksamen/vurdering

Laboratory exercises, Projects with mandatory handing in of reports

Mandatory lab assignments are to be completed at the times and in the groups that are assigned and published. Absence due to illness or other reasons must be communicated to the laboratory personnel as soon as possible. One cannot expect that provisions for completion of the lab assignments at other times can be made, unless prior arrangements with the laboratory personnel have been agreed.

Failure to complete the assigned labs on time or not having them approved will result in barring from taking the course exam.

Fagperson(er)

Coordinator laboratory exercises:

Liv Margareth Aksland

Coordinator laboratory exercises:

Dugassa Nemie-Feyissa

Head of Department:

Päivi Annele Teivainen-Lædre

Study Program Director:

Mark van der Giezen

Course coordinator:

Federico Fenaroli

Method of work

2 hours lectures/laboratory review and 12 hours laboratory work and lab reports for 8 and 6 weeks, respectively. Mandatory work demands (such as hand in assignments, lab- assignments) must be approved by the course teacher three weeks before the examination date.

Overlapping

Emne Reduksjon (SP)
Methods in Biotechnology (BIO520_1) , Biotechnology, methods (MOT380_1) 10

Åpent for

Master level studies at the Faculty of Science and Technology

Emneevaluering

Forms and/or discussion

Litteratur

Compendium in biotechnology. Biology, Campbell & Reece, selected chapters.

Kontakt

Coordinator laboratory exercises:

Liv Margareth Aksland

Coordinator laboratory exercises:

Dugassa Nemie-Feyissa

Head of Department:

Päivi Annele Teivainen-Lædre

Study Program Director:

Mark van der Giezen

Course coordinator:

Federico Fenaroli

Tilbys av

Faculty of Science and Technology

Department of Chemistry, Bioscience and Environmental Engineering

The course description is retrieved from FS (Felles studentsystem). Version 1